RESUMEN
Cervical cancer is the fourth most common cancer among women worldwide. The main factor associated with the onset and progression of this neoplasia is the human papillomavirus (HPV) infection. The HPV-oncogenes E6 and E7 are critical drivers of cellular transformation, promoting the expression of oncogenes such as KCNH1. The phytochemical α-mangostin (AM) is a potent antineoplastic and antiviral compound. However, its effects on HPV oncogenes and KCNH1 gene expression remain unknown. This study evaluated the effects of AM on cell proliferation, cell cycle distribution and gene expression, including its effects on tumor growth in xenografted mice. AM inhibited cell proliferation in a concentration-dependent manner, being the most sensitive cell lines those with the highest number of HPV16 copies. In addition, AM promoted G1-cell cycle arrest in CaSki cells, while led to cell death in SiHa and HeLa cells. Of interest was the finding of an AM-dependent decreased gene expression of E6, E7 and KCNH1 both in vitro and in vivo, as well as the modulation of cytokine expression, Ki-67, and tumor growth inhibition. On these bases, we suggest that AM represents a good option as an adjuvant for the treatment and prevention of cervical cancer.
Asunto(s)
Proteínas Oncogénicas Virales , Infecciones por Papillomavirus , Neoplasias del Cuello Uterino , Humanos , Femenino , Animales , Ratones , Neoplasias del Cuello Uterino/tratamiento farmacológico , Neoplasias del Cuello Uterino/genética , Neoplasias del Cuello Uterino/patología , Proteínas Oncogénicas Virales/genética , Proteínas Oncogénicas Virales/metabolismo , Células HeLa , Proteínas E7 de Papillomavirus/genética , Proteínas E7 de Papillomavirus/metabolismo , Proteínas Represoras/genética , Oncogenes , Proliferación Celular , Expresión Génica , Canales de Potasio Éter-A-Go-Go/genéticaRESUMEN
Este trabajo aborda los efectos del Covid-19 en el circuito punk de Chapinero. Utilizamos el concepto de circuito para operacionalizar el análisis, caracterizando los espacios de la oferta cultural y los consumidores que participan en ellos, notables por su movilidad, que aproximamos desde la territorialidad nómada. Examinamos los efectos económicos y políticos mediante entrevistas a los gestores y encuestas a los consumidores. Los resultados presentan la cartografía del circuito punk de Chapinero, así como las transformaciones en las formas de producir y de consumir. Encontramos que tanto los productores como consumidores no han podido continuar con su participación en el circuito al existir elementos de socialización inherentes al encuentro en los espacios físicos. Los espacios han afrontado la reducción de sus ingresos recurriendo a la solidaridad y apoyo mutuo. En términos políticos, la falta de espacios de encuentro y deliberación parece limitar la naturaleza contracultural del circuito.Alternate abstract:In this paper we analyze the effect of Covid-19 on the Chapinero punk circuit. Our analysis operationalizes the concept of circuit for it characterizes the fluidity observed in the spaces and participants. We approach this character from the perspective of nomad territoriality. We focus on the economic and political effects, analyzing them through interviews with the producers and a consumer survey. We present a cartography of the Chapinero punk circuit and describe the transformations that have taken place in the production and consumption. We find that both the producers and consumers have not been able to continue participating in the circuit, since there are elements inherent to the use of physical spaces and their socialization. The spaces have faced lower revenues, which they have supplemented through solidarity and mutual aid. The lack of meeting and deliberation spaces seems to limit the countercultural nature of the circuit.